【文獻標題】Prostaglandin Metabolome Profiles in Zebrafish (Danio rerio) Exposed to Acetochlor and Butachlor 【作者】Shenggan Wu , Xinzong Zhou , Weiwei Qin et.al 【作者單位】浙江農業科學院（Zhejiang Academy of Agricultural Sciences） 【文獻中引用產品】 斑馬魚前列腺素（PGs）ELISA試劑盒 斑馬魚前列腺素E2（PGE2）ELISA試劑盒 斑馬魚前列腺素D2（PGD2）ELISA試劑盒 斑馬魚13,14二氫15-酮前列腺素F2α(PGFM)ELISA試劑盒 斑馬魚活性氧（ROS）ELISA試劑盒 【關鍵詞】prostaglandins; metabolites; isoprostanes; Danio rerio; UPLC–ESI–MS/MS-metabolomics 【DOI】doi.org/10.3390/ijms24043488 【影響因子(IF)】5.59 【出版期刊】《International Journal of Molecular Sciences》 【產品原文引用】 The samples, mixed with eight females or males per repeat, were crushed and homogenized in the Phosphate Buffered Saline (PBS) solution (0.01 M, pH 7.4). Three repeat samples were used for each treatment. The homogenate was centrifuged at 5000 rpm/min for 10 min and the supernatant was separated. The collected supernatant was then stored at 80 C. The levels of the total PGs, PGE2, PGD2, 11β-13,14-dihydro-15-keto PGF2α (PGFM), 5-iPF2a-VI, and ROS were detected by commercial ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions. These kits employed HRP-conjugate mouse antibodies to quantify PGs and ROS levels in samples. Statistical differences were subjected to one-way analysis of variance (ANOVA), followed by Tukey’s multiple comparison test. The statistical significance level was set at p < 0.05.
完整版PDF文獻請咨詢在線客服或者聯系我司業務員！ 更多公司福利請關注“恒遠生物"微信公眾號！
|
點擊交流